Enzymatic Hydrolysis of Liquid Hot Water Pre-treated Macroalga (Ulva lactuca) for Fermentable Sugar Production

Mahmudi, Ali (2017) Enzymatic Hydrolysis of Liquid Hot Water Pre-treated Macroalga (Ulva lactuca) for Fermentable Sugar Production. In: The 24th Regional Symposium on Chemical Engineering (RSCE 2017), November 15-16, 2017, Semarang, Indonesia. (Submitted)

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Abstract

Ulva lactuca is one of green macro-algae that has a significant cellulose content. This study aims to determine the effect of variations in substrate-enzyme ratio and hydrolysis time on the enzymatic hydrolysis process of cellulose extracted from Ulva lactuca to produce fermentable sugar or reducing sugar as a raw material for making bioethanol. Firstly, Liquid Hot Water (LHW) pre-treatment process was performed at the temperature of 135oC for 20 minutes; the purpose of this pre-treatment was to reduce the content of hemicellulose and to increase the cellulose content. Secondly, enzymatic hydrolysis process using cellulase enzyme was carried out, in this process citrate buffer was needed in order to stabilize the pH level during hydrolysis process. The process variables were ratio of substrate-enzyme (1:0.05; 1:0.1; 1:1.5; 1:2 and 1:2.5 w/w) and hydrolysis time (24, 48 and 72 hours) under temperature of 45oC and pH level of 5.5. The results shows that the highest reducing sugar yield is 79.7% obtained at a ratio of substrate-enzyme of 1:2.5 (w/w) for 48 hours of hydrolysis time, with the result of reducing sugar concentration is 16.2043 mg/mL.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Enzymatic Hydrolysis
Subjects: Engineering > Informatics Engineering
Divisions: Fakultas Teknologi Industri > Teknik Informatika S1
Depositing User: Mrs Retno Wulan Sari
Date Deposited: 24 Aug 2023 03:29
Last Modified: 24 Aug 2023 03:29
URI: http://eprints.itn.ac.id/id/eprint/12677

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